Leaf culture inoculation method (IMAGE) American Phytopathological Society Caption Leaf culture inoculation method for Cercospora kikuchii. Leaflets were placed in a disposable plastic case and kept in high humidity for 9 days to promote infection. A, Picture of the plastic case with soybean leaflets. B, Leaf pieces after spray inoculation. Credit Takeshi Kashiwa, Miguel Angel Lavilla, Antonio Diaz Paleo, Antonio Juan Gerardo Ivancovich, and Naoki Yamanaka Usage Restrictions Credit must be given to the creator. License CC BY Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.