Figure 1 (IMAGE)
Caption
The Cdkn1a variant 2 transcript is preferentially induced during aging. (A) We designed primers (black arrows) to specifically detect Cdkn1a variant 1 and 2 transcripts, spanning the first and second exons in each case. The protein-coding region is indicated as well as the ATG start codon (brown arrow). Transcription starts at +1 for p21var1 and at -2790 for p21var2. The first and last bases of each exon are also indicated. (B) mRNA levels of p21var1 and p21var2 in the livers of male mice aged 2 to 30 months of age, normalized to levels in livers of 2 month-old animals. (C-G) Levels of each Cdkn1a transcript were assessed in 2 (young) and 24 (old) month-old mice. Animals were young males (n = 12), young females (n = 12), old males (n =14-15) and old females (n = 14-15). Results are shown for (C) liver, (D) adipose tissue, (E) kidney, (F) heart and (G) lung. In (C-G) data were normalized to p21var1 levels in young mice. Note Y axes are log-2 scales. 1-way ANOVA and Tukey post-tests were applied. * p < 0.05, ** p < 0.01, *** p < 0.001, ns = not-significant.
Credit
Correspondence to: Judith Campisi email: jcampisi@buckinstitute.org
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Copyright: © 2021 López-Domínguez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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