Figure 5 (IMAGE)
Caption
IFNβ-induced AML-cell cytotoxicity is enhanced with anti-CD38 antibody daratumumab. (A-C) AML cell lines (MV-411, n = 9 experiments and OCI-AML3, n = 7 experiments) and primary AML apheresis samples (n = 3) were treated with or without 1000 U/mL IFNβ for 24 h and then incubated with opsonized sheep red blood cells. Phagocytosis was evaluated via microscopy in a blinded fashion. The phagocytic index represents the number of red blood cells ingested by 100 AML cells for each respective cell line. (D) OCI-AML3 cells (n = 7 experiments) were treated with or without anti-CD38 antibody (α-CD38, 20 μg/mL), IFNβ (500 U/mL), or α-CD38 + IFNβ for 48 hours. Cytotoxicity was then measured using a Lactate Dehydrogenase Assay. (E and F) MV4-11 cells (n = 6 experiments) were treated with IFNβ (500 U/mL) for 48 hours. Cells were then plated on methocult™-media-containing plates for 10 days, then colonies counted in a double-blinded fashion. (G) Current working model, as described in the text. *p ? 0.05.
Credit
Correspondence to - Jonathan P. Butchar - butchar.2@osu.edu and Susheela Tridandapani - tridandapani.2@osu.edu
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Copyright: © 2021 Fatehchand et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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