Figure 1 (IMAGE) Impact Journals LLC Caption Pharmacological inhibition of Bcl-2 and/or Bcl-xL increases Mcl-1 expression through cap-dependent translation. (A) Relative MCL1 transcript levels were determined by RT-qPCR after treatment with 1.0 μM ABT-263 for 16 hrs. Values were standardized to DMSO control for each cell line. Each data point represents the average of three technical replicates, midlines are the average of the biological replicates. P-value calculated using Student's unpaired two-tailed t-test. (B-C) Western analysis of lysates from cells treated with 1.0 μM ABT-263 for 16 hrs then chased with cyclohexamide (CHX) for 0-30 minutes. (B) Representative images are shown. Antibodies used are shown to the left of each panel. (C) Average Mcl-1 band density (± S.E.) is shown, N = 3. Two-way ANOVA and Bonferroni post hoc test. (D-F) Western analysis of whole cell lysates were harvested from cell treated with 1.0 μM ABT-263, ABT-199, A-1155463, or ABT-199 + A-1155463 for 16 hrs. Where indicated, 5.0 μM 4E1RCat was added for the final 4 hrs. Antibodies used are shown to the left of each panel. Credit Correspondence to - Rebecca S. Cook - Rebecca.cook@vanderbilt.edu Usage Restrictions ccby License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.