Figure 5 (IMAGE)
Caption
Experiment design for clonal assay in T75 flasks in cell lines treated with sirolimus at different time and concentration. Experiments parameters used were: - synchronized colonies, no use of CO2, fructose based media, - antibiotics free (streptomycin/penicillin) - long term exposition to sirolimus (5 weeks) - cells are grown for 1 months before any treatment - doubling time was constant for cell lines so growth of cells is predictable - monodisperse of cells
Credit
Correspondence to - Gianandrea Pasquinelli - gianandr.pasquinelli@unibo.it
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