Figure 6 (IMAGE)
Caption
TCGA analyses (expression, metastasis and prognosis) and possible CD98hc-independent cell-surface expression of LAT1 in human cells. TCGA data (A-C) were obtained from the UCSC Xena browser. (A) Box-whisker plot of the expression of CD98hc (right) /LAT1 (left) in normal and tumor colorectal tissues. P-values were calculated by the Student's t test. (B) Box-whisker plot of the expression of LAT1 in non-metastatic and metastatic ccRCC. The presence of metastasis was determined based on pathological TMN classification. P-values were calculated by the Student's t test. (C) Kaplan-Meier survival analysis with the log rank test of ccRCC and bladder cancer patient. The correlation between mRNA expression (top 25% and lower 25%) and survival was evaluated. (D) Effects of CD98hc-KO on the expression of LAT1. CD98hc in HE293F and SW1116 cells was disrupted by the CRISPR/Cas9-based method, and the expression of CD98hc and LAT1 was analyzed by FCM. (E) WB analysis of LAT1 protein under reducing or non-reducing conditions using various human cell lines. Lysates were subjected to SDS-PAGE under reducing or non-reducing conditions, and to WB with anti-LAT1 rabbit pAb.
Credit
Correspondence to - Takashi Masuko - masuko@phar.kindai.ac.jp
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Copyright: © 2021 Hayashi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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