Figure 4 (IMAGE)
Caption
GLI1 protects CLL cells from apoptosis. (A) CLL cells were transfected with GLI1-siRNA or with an empty vector marked as CTRL (left bar). The comparative CT method was used to compare the transcript levels of GLI1, STAT3 and GLI1-regulated genes Forkhead box M1 (FOXM1), Platelet-derived growth factor receptor-α (PDGRFA) and Relaxin family peptide receptor 2 (RFPR2). *** denotes P < 0.0001, ** denotes P < 0.001, * denotes P < 0.01, and NS denotes statistically not significant compared to control. (B) Flow cytometry analysis of CLL cells that were transfected with GLI1-siRNA or with GAPDH (transfection control). Apoptosis rate was assayed double staining with annexinV/PI. As shown the rates of apoptotic cells increased by approximately than 3-fold. (C) The apoptosis rates of CLL cells incubated overnight with or without 10 μM of the GLI1 inhibitor GANT61 are shown. Depicted are data from 3 different experiments.
Credit
Correspondence to - Zeev Estrov - zestrov@mdanderson.org
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Copyright: © 2021 Rozovski et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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