MPMI Focus Issue Cover (IMAGE) American Phytopathological Society Caption The potyviral protein CI protein forms cone-shaped structures at the plasmodesmata (PD) to assist in potyviral intercellular movement. The deposition of the cone-shaped structures of CI to PD is directed by the potyviral PD-located protein P3N-PIPO through its interaction with CI. The confocal microscopy image shows localization of CI-mRFP expressed in a leaf cell of Nicotiana benthamiana infected by turnip mosaic virus (TuMV). The plasma membrane (PM) was labelled by coexpression of the PM marker GFP-REM. Such typical subcellular localization of CI was observed only when CI was coexpressed with P3N-PIPO but not with any other TuMV proteins. The image was taken 48 h postagroinfiltration Credit Taiyun Wei and Aiming Wang Usage Restrictions None License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.