HeLa Cell Expressing Fluorescent GEM Particles (IMAGE)

IMBA- Institute of Molecular Biotechnology of the Austrian Academy of Sciences

HeLa Cell Expressing Fluorescent GEM Particles

Caption

Images of a live HeLa cell expressing fluorescent GEM particles (green) to probe the localization of large cytoplasmic components during cell division. Chromosomes are fluorescently labelled in magenta. During early stages of mitotic exit (image on the left side), chromosomes are still far apart, and GEM particles localize throughout the cell and also in spaces between chromosomes. Later in mitosis, as the chromosomes move towards the cell poles, they come together to form a dense cluster (image on the right side). During this clustering process, the GEM particles are excluded from the space between chromosomes. The nuclear envelope, which subsequently wraps around chromosomes then does not incorporate cytoplasmic GEMs and potentially other cytoplasmic contaminants.

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©Gerlich lab/IMBA

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