Time-lapse STED Imaging of Cristae Mergence inside a Single Mitochondrion (VIDEO)
Caption
Video 1. Live mitochondria imaged in unprecedented detail -- for an unprecedented length of time -- using the MitoPB Yellow fluorescent marker created by Nagoya University-led researchers. The marker molecule is designed to be absorbed by only certain membranes within each mitochondrion, and retains its fluoresescence under the STED microscope for a very long time. This video was shot at 1.5 fps and a resolution of 90nm. Still images were captured at 60nm resolution. In response to being deprived of nutrients, mitochondria fuse together and increase the number of cristae. This time-lapse sequence shows events such as two separate mitochondria fusing together to form a single mitochondrion; and a single mitochondrium fusing together. Note that the outer membranes of the mitochondria are invisible: we are seeing the inner membranes fusing together. More videos and images can be viewed at the paper's PNAS website. https://www.pnas.org/content/116/32/15817/tab-figures-data
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© ITbM, Nagoya University
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