Differential cell permeability time lapse movie (VIDEO)

University of Nottingham

Caption

Illustrates that differential cell permeability is the key to creating the exogenous-GA-generated gradient. Technical detail: The time-course shows a root treated with nlsGPS1 growing in Rootchip18S with GA at standard (pH 5.7) then low pH (pH 4.5). At pH 5.7 (8s of the video) the exogenous GA4 accumulated faster in the elongation zone, but at low pH (pH 4.5) (16s of the video) a fast and strong accumulation of exogenous GA4 also occurs in the meristematic zone suggesting that raised apoplastic pH in the meristematic zone limits exogenous GA4 accumulation.

Credit

Sainsbury Lab, University of Cambridge and University of Nottingham

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