A New Vector for Gene Transfer across the Blood Brain Barrier (VIDEO)
Caption
Using a new selection method reported in Nature Biotechnology, Caltech researchers have evolved the protein shell of a harmless virus, AAV9, so that it can more efficiently cross the blood brain barrier and deliver genes, such as the green fluorescent protein (GFP) to cells throughout the central nervous system. This movie shows green fluorescent protein (GFP) expression in the spinal cord of an adult mouse that received an intravenous injection of the modified virus, AAV-PHP.B, carrying the gene for GFP. The spinal cord sample was rendered transparent for imaging using a whole-body tissue clearing method called PARS CLARITY previously developed by the Gradinaru lab.
Credit
Ben Deverman and the Gradinaru laboratory/Caltech and Nature Biotechnology
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