Harmonic Generation Microscopy (IMAGE)

Optica

Harmonic Generation Microscopy

Caption

This series of harmonic generation microscopy images shows the skin cells of a 24-year-old subject at increasing depths, ranging from the outermost layer of skin (a) to approximately 300 millionths of a meter deep (f). The magenta areas, generated from third harmonics, show skin cells and their nuclei. The green areas, generated from second harmonics, show fibers made of the protein collagen.

Credit

<i>Biomedical Optics Express</i>

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