Schematic Illustration of Fluorescence-Based Aldolase Assay (IMAGE)

SLAS (Society for Laboratory Automation and Screening)

Schematic Illustration of Fluorescence-Based Aldolase Assay

Caption

ALDOA catalyzes the reversible aldol cleavage of fructose-1,6-bisphosphate (F-1,6-BP) into D-Glyceraldehyde-3-phosphate (GAP) and dihydroxyacetone phosphate (DHAP). ALDOA activity is assayed by monitoring NADH levels via a coupled enzymatic reaction using Glycerophosphate Dehydrogenase (alpha-GDH) and NADH, where GDH converts DHAP to L-alpha-Glycerol phosphate (GP) with the concomitant oxidation of NADH to NAD+. Elite NADH detection kit reduces the probe into a highly fluorescent product that is excited at 540-570 nm.

Credit

Eun Jeong Cho, Ph.D.

Usage Restrictions

None

License

Licensed content

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.