Sesaminol prevents Parkinson's disease by activating the Nrf2-ARE signaling pathway (IMAGE)
Caption
(A) The neuroprotection by sesaminol. ARE: antioxidant response element, EpRE: electrophile responsive element, ROS: reactive oxygen species, NQO1: NAD(P)H: quinone oxidoreductase, HO-1: hemo oxygenase-1, γ-GCS:γ-glutamylcysteine synthetase
(B) Effects of sesaminol and 6-OHDA on the viability of SH-SY5Y cells. SH-SY5Y cells were incubated with 0.25~10 μg/ml of sesaminol for 2 h, followed by treatment with 20 μM 6-OHDA for 24 h. The cell viability was measured using the MTT assay. The results represent the means ± SD of 6 experiments. Values without a common letter are significantly different (p<0.01).
(C) Effects of 6-OHDA and sesaminol on nuclear translocation of Nrf2. SH-SY5Y cells were incubated with 1 μg/ml of sesaminol for 2 h, followed by treatment with 20 μM 6-OHDA for 3 h. Intracellular Nrf2 was stained using primary and secondary antibodies and nuclei were stained using DAPI.
(D) Effects of 6-OHDA and sesaminol on intracellular ROS levels. SH-SY5Y cells were incubated with 1 μg/ml of sesaminol for 2 h, followed by treatment with 20 μM 6-OHDA for 6 h. Intracellular ROS levels were measured using DCFH-DA. The fluorescence intensity of intracellular ROS. The results represent the means ± SD of 10 cells. Values without a common letter are significantly different (p<0.05).
Credit
Akiko Kojima-Yuasa
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