Time-Lapse Movie of Aging (VIDEO)
Caption
Right, movies of representative mode 1 (top) and mode 2 (bottom) aging cells (encircled) are played sequentially. Green and red fluorescence reflect nucleolar and mitochondrial integrity in the cells during aging. Increase in green fluorescence represents loss of nucleolar integrity whereas decrease in red fluorescence represents loss of mitochondrial integrity. Left, real-time quantification of fluorescence plotted within a 3D aging space, in which z-axis represents the percentage of lifetime. After the aging trajectories of the two representative cells were quantified and plotted, trajectories of a population of isogenic wild type cells were plotted in the space (mode 1: red; mode 2: blue).
Credit
Hao Lab, UC San Diego
Usage Restrictions
Must be used in conjunction with Science paper on aging.
License
Licensed content