Up-regulation of IRF8 in the liver of mice and humans with NAFLD. (IMAGE)
Caption
(A–H) Western blots and quantitative PCR were performed to analyze IRF8 levels in the livers of C57BL/6J male mice fed with HFD or CD (A, B) (n = 5 biologically independent mice), lean or ob/ob male mice (C, D) (n = 5 biologically independent mice), C57BL/6J male mice fed with HFHC (40 kcal of fat, 20 kcal of fructose, and 2% cholesterol) or CD for 30 weeks (E, F) (n = 5 biologically independent mice), and lean or db/db male mice (G, H) (n = 5 biologically independent mice). (I, J) After culturing MPHs using DMEM plus 10% fetal bovine serum for 24 h, the relative protein and mRNA expression levels of IRF8 were measured. The remaining cells were further cultured in DMEM for another 24 h to detect the expression level of IRF8. Subsequently, the remaining cells were cultured in DMEM plus 10% fetal bovine serum for an additional 24 h to evaluate the relative protein and mRNA expression levels of IRF8. (K, L) MPHs were treated with 5% bovine serum albumin, 0.1 mM palmitic acid (PA), and 0.25 mM PA for 24 h, and then the mRNA (K) and protein (L) levels of IRF8 were examined. (M) IRF8 protein levels were analyzed in liver tissues from healthy or patients with NAFLD by western blots (n = 5 biologically independent individuals). Data are presented as mean ± standard error of the mean. p values were calculated using an unpaired two-tailed Student's t-test (B, D, F, H, J–L). IRF8, interferon regulatory factor 8; NAFLD, non-alcoholic fatty liver disease; HFD, high-fat diet; CD, chow diet.
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Genes & Diseases
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