Ethanol and burn injury alter relative abundance of bacterial phyla in the small intestine and caecum. (IMAGE)
Caption
Small intestinal and caecum luminal faecal contents were collected 1 day after ethanol and burn injury, and faecal bacterial DNA was isolated for 16S sequencing. Schematics represent the abundance (read count) of individual bacterial phyla relative to total bacterial read counts and averaged across the groups depicted. (A) Parts of a whole plot of SV and EB small intestinal faecal phyla, respectively. (B) Bar graphs comparing small intestinal SV and EB with male and female samples separated. (C) Parts of a whole plot of SV and EB caecum faecal samples phyla respectively. (D) Bar graphs comparing caecum SV and EB with male and female samples separated. (A, C) Parts of a whole chart combine male and female samples together to compare SV to EB overall. Significance was analysed by Mann-Whitney U test, n=10–13 animals per group, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. (B, D) Statistical analysis via non-parametric Kruskal-Wallis test (significance shown in colour legend) with on graph significance depicting Dunn’s multiple comparisons test with associated male or female SV samples, n=5–7 animals per group, *p<0.05, **p<0.01. EB, ethanol burn; SV, sham vehicle.
Credit
By Caroline J Herrnreiter, Mary Grace Murray, Marisa Luck, et al.
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License
CC BY-NC