Deciphering age-related transcriptomic changes in the mouse retinal pigment epithelium (IMAGE)
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Figure 1. Global gene expression analysis in RPE/choroid of young and aged mice. (A) Pie chart represents the percent of globally expressed transcript subspecies across young (2–3 months, n = 4) and aged (22–24 months, n = 4) mice RPE/choroid from 21,376 annotated transcripts. (B) PCA plots of whole transcriptome data showed distinct clustering of young and aged mice along PC1, which captures the maximum variance (52.4%). Blue and red ellipses indicate young and aged mice and black dots represent the biological replicates (n = 4). (C, D) GO enrichment terms associated with the top 100 PC1 positive loading genes (C) and PC1 negative loading genes (D). (E) MA plot of log2 fold change versus average log counts-per-million (CPM) based on edgeR analysis showing the differential gene expression between aged and young mice RPE/choroid. Red dots indicate differentially expressed genes (FDR ≤0.05), and black dots indicate non-differentially expressed genes. (F) Volcano plot showing genes significantly (p-adj value < 0.05) upregulated (red) and downregulated (blue) in aged mice RPE/choroid. The x-axis represents log2-fold change, and the y-axis represents −log10 (p-value). The dotted line shows a cutoff of −log10 (p-value) < 0.05. Annotated dots represent the top significantly regulated genes. (G) Hierarchical clustering and heatmap analysis of gene expression in the RPE/choroid tissues of the young vs. aged mice. Blue to red represents low to high gene expression.
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Copyright: © 2025 Dubey et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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