Experimental design and methods overview. (IMAGE)
Caption
Experimental design and methods overview. a Protocol timeline. b The process of cell aggregation and organoid formation. The dotted line represents the organoid edge. Scalebar = 200 µm. c organoid fusion leading to the formation of macro-scale assembloids. d Alcian blue staining of cartilaginous extracellular matrix (ECM) of an assembloid after 24 h, and (e) safranin-o/fastgreen staining of sulphated glycosaminoglycans (sGAG). Scalebar = 500 µm. f Experimental design to assess the requirements for cartilage-to-bone transition. For chondrogenic treatment, cells from 10 donors were evaluated (5 male, 5 female, aged 16–29). As undifferentiated control, 5 donors were evaluated (3 male, 2 female, age 21–29).
Credit
Bone Research
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