FGR Src family kinase causes signaling and phenotypic shift mimicking retinoic acid-induced differentiation of leukemic cells (IMAGE)
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Figure 6: Cell cycle analysis of HL-60 WT and FGR O.E cells.
DNA histograms show FGR transfectants were enriched for relative number of G1/0 cells compared to the wt cells. Wild-type and FGR O.E cell lines were cultured for 72 h without (untreated control) or with 1 μM RA as indicated. Cell cycle distribution showing the percentage of cells in G1/G0 was analyzed using flow cytometry with propidium iodide staining at 72 h. Gates define the G1, S, and G2/M subpopulations (left to right). G1/0 arrest is indicated by an increase in the G1 peak for FGR O.E compared to wt cells. Quantification of 3 or more biological repeats is in Supplementary Figure 22.
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