The relative expression of SOX9, Wnt/β-catenin signaling pathway and epithelial-mesenchymal transition markers in hypospadias foreskin tissues. (IMAGE)
Caption
Relative expression of SOX9, Wnt/β-catenin signaling pathway and epithelial-mesenchymal transition markers in hypospadias foreskin tissues. (A) The protein expressions of SOX9, Wnt3a, β-catenin, LEF1, GSK3β, NF-κB were measured by Western blotting. (B) Detection of SOX9, Wnt3a, β-catenin, TCF1, c-Myc, cyclin D1 mRNA levels (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). (C) Immunofluorescence showed that the expression of SOX9 in Hypospadias foreskin group was significantly lower than that in Normal foreskin group(fluorescence microscope × 200). (D) Western blotting analyses of α-SMA, Vimentin, E-cadherin, Occludin and β-catenin protein levels of tissue specimens. (E) Detection of α-SMA, Vimentin, E-cadherin, Occludin and β-catenin mRNA levels (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
Credit
Pediatric Discovery
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