The level of STIM1 is elevated in SR-HCC cell models. (IMAGE)

Chongqing Medical University

The level of STIM1 is elevated in SR-HCC cell models.

Caption

The level of STIM1 is elevated in SR-HCC cell models. (A) CCK-8 assays were used to examine the cell viability of sorafenib-parental (Con) and -resistant (SR) Hep3B and MHCC97H cells treated with various doses of sorafenib for 72 h. (B) Real-time quantitative PCR and (C) western blotting were applied to evaluate the mRNA and protein expression of STIM1 in Con- and SR-Hep3B and MHCC97H cells. β-actin was used as a loading control. (D) Calcium imaging was applied to analyze the calcium mobilization in Con- and SR-Hep3B and MHCC97H cells, respectively upon cyclopiazonic acid (CPA) (200 μM) stimulation. (E) The heatmap indicating the top 100 up-regulated and down-regulated genes between Con- and SR-Hep3B cells as detected in RNA next-generation sequencing with three biological duplicates. (F) Differentially expressed genes between Con- and SR-Hep3B cells were analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. (G) GSEA demonstrated the correlation between SR and calcium signaling pathway in Hep3B SR cells. Data were expressed as mean ± standard error of the mean. ∗∗P < 0.01, ∗∗∗P < 0.001.

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Genes & Diseases

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