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Caption
Overview of the sound delivery system for assaying C. elegans auditory responses. A Schematic describing the custom sound delivery system used to examine worm auditory responses. The sound generator (here, computer) is connected to a speaker mounted on a micromanipulator. This enables precise adjustments of the speaker opening towards the target. The speaker output port is fitted with a short length of 1/8” PVC tubing to restrict the speaker opening size, which can be further modified via the attachment of a shortened p2 pipette tip (trimmed to the desired opening size) to the open end of the tubing. The system is designed for use with a stereomicroscope to monitor worm behavior. The inset depicts the recommended distance and angle for positioning the speaker opening towards the worm. B Snapshot image showing the sound delivering system positioned above the stereomicroscope field of view. The micromanipulator base is mounted on a wooden block to facilitate positioning the speaker at the base of the stereomicroscope field of view. C Inset from Panel B, depicting a zoomed-in snapshot image of the speaker tip positioned above the NGM testing plate. The speaker tip is held 1 mm above the NGM surface at a 45° angle
Credit
Can Wang, Elizabeth A. Ronan, Adam J. Iliff, Rawan Al-Ebidi, Panagiota Kitsopoulos, Karl Grosh, Jianfeng Liu, X.Z. Shawn Xu
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