PULSE: an acoustic-based automated single-cell-analysis platform. (IMAGE)
Caption
PULSE: an acoustic-based automated single-cell-analysis platform. a Biofabrication via PULSE from the microscale to the macroscale. n, particle number. b Precision gating via PULSE for single-cells and bulk-cells. c Deterministic array barcoding of known cells via known primers. d Schematic overview of the dispensing module of the PULSE platform. “myBase”: homemade software for controlling PULSE over various parameters in vector E. Scale bar: 1 cm. e A PULSE cartridge integrating an acoustic-based multicolor single-cell ejector and three independent reagent ejectors on a piezoelectric substrate. A circularly confined ring disc is placed over the ejector to constrain the liquid meniscus and dampen the water waves. f Aligned printing of nanodrops into microwells. Inset: an ejected droplet containing one fluorescent particle. Scale bar: 200 µm. g PULSE deterministically threads the whole pipeline of quantitative precision biological experiments with single-cell resolution, from experimental design, parameter matrix formation, and nanodrop printing to acquiring single-cell response and sequencing data on a compact device. [c, d]: c, the vector of cell types and numbers; d, the vector of drug types and concentrations. “n-D”, n-dimensional.
Credit
Microsystems & Nanoengineering
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CC BY