Leukopenia, weight loss and oral mucositis induced by 5-Fluorouracil in hamsters’ model: A regenerative approach using electrospun poly(Lactic-co-Glycolic Acid) membrane (IMAGE)
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Figure 7: Timeline diagram showing cell isolation and proliferation on PLGA.
Day 1, excision of the jugal cheek pouch and cell isolation (A, B); day 2, cell proliferation in the culture dish, such as represented by the cells in an amplified image in (C) (Nikon/ ECLIPSE Ti-S/objective 20X); day 3, addition of 3 × 105 cells on the PLGA membrane; days 4, 5, 6, 7, the cells were proliferating on the PLGA membrane (D, E - Cell Tracker-red/Molecular Probes Life Technologies/lot 1756851/Nikon/ECLIPSE Ti-S/objective 20X); day 7, the PLGA dressing with or without cells were applied to the ulcerative lesions as dressings for 6 days; day 13, the dressing was removed and the ulceration region was excised for optical microscopy analysis. Scale bars: 100 µm.
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