Robust p53 phenotypes and prospective downstream targets in telomerase-immortalized human cells (IMAGE)
Caption
Figure 8: Regulation of ALDH3A1 and NECTIN4 by p53.
(A) The indicated tracks showing H3K27Ac density data on 7 cells lines from ENCODE, ChIP-seq density and p53-specific ChIP-seq data from ReMap and putative transcription start sites identified via CAGE by the FANTOM5 consortium are shown below a schematic of the ALDH3A1 locus. An upstream p53 ChIP-seq peak is indicated by the green arrow; the associated TSS is indicated by a red arrow. (B) A putative p53 response element was found -199 bp upstream of the TSS. (C) RT-qPCR analysis of relative ALDH3A1 expression across the hTERT-RPE1 cell panel. (D) The NECTIN4/NECTIN4-AS1 locus aligned to regulatory elements, as in (A). p53 ChIP-seq peaks are indicated by green arrows. (E) RT-qPCR analysis of relative NECTIN4 and NECTIN4-AS1 expression across the hTERT-RPE1 cell panel. (F) Relative expression of NECTIN4 and ALDH3A1 in p53WT/p53MUT isogenic lines in the CRC panel described in Figure 1, downloaded from (GSE13886). The units on the Y-axis represent fold-induction. The RT-qPCR results shown are representative of a minimum of two independent experiments.
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