The effects of EFC on RA FLSs, PBMCs, and THP-1 cells (IMAGE)
Caption
RA FLSs, PBMCs, and THP-1 cells were treated with vehicle control and different concentrations of EFC for 72 h (a) or 24 h (b). (a) The cell number was measured using the CCK-8 assay, and IC50 values were derived. (b) Total RNA was collected, and the mRNA levels of IL-6, IL-8, NOS2, and COX2 were measured by RT-qPCR. Data are presented as mean ± standard deviation (SD). **p < 0.01, ***p < 0.001. CCK-8, Cell Counting Kit-8; COX, cyclooxygenase; EFC, E64FC26; FLSs, fibroblast-like synoviocytes; IC50, inhibitory concentration 50; IL, interleukin; IFN-γ, Interferon-gamma; LPS, lipopolysaccharide; NOS, nitric oxide synthase; PBMCs, peripheral blood mononuclear cells; RA, rheumatoid arthritis; RT-qPCR, real-time quantitative polymerase chain reaction; THP-1, human monocytic leukemia cell line.
Credit
Jinxiang Han, Lin Wang, Haiyan Zhao, Ting Wang
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License
CC BY-NC