Graphical Abstract of the Research Protocol (IMAGE)

University of Liège

Graphical Abstract of the Research Protocol

Caption

The semen was collected and then analysed for viability. A portion was inseminated immediately, while the rest was frozen. The viability of the thawed semen was also analysed. The thawed semen was then inseminated. In total, sixteen queens received thawed semen, while thirteen received freshly collected semen as a control group. Three queens from the control group produced female brood, whereas five queens from the thawed semen group produced female offspring.

Graphical abstract created using BioRender. Egyptien, S. (2025) https://BioRender.com/i71j767.

Credit

University of Liège / Sophie Egyptien

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