B16F10 Cell-Induced Osteocyte Ferroptosis in Bone Metastasis. (IMAGE)
Caption
B16F10 Cell-Induced Osteocyte Ferroptosis in Bone Metastasis. a H&E staining of cortical bone from control and B16F10-injected mice (n ≥ 6). White arrows indicate normal osteocytes; green arrows, dying osteocytes; and red arrows, dead osteocytes. Scale bars: 20 μm. b TUNEL staining of tibial bone sections from control and B16F10-injected mice (n = 5). White arrows mark TUNEL-positive cells. Scale bars: 20 μm. c mRNA expression of Dmp1, Dkk1, Phex, Sclerostin, Col1a1, and Runx2 in long bone (without bone marrow) from control and B16F10-injected mice (n ≥ 6). d PCA plot showing sample patterns of individual samples in the control and B16F10 groups. e KEGG pathway analysis indicating “Ferroptosis” as the significantly altered pathway. f Heatmap of differentially expressed genes in ferroptosis pathways. Statistical significance was determined by a 2-tailed Student’s t-test (A, B, C).
Credit
Bone Research
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CC BY