Human induced pluripotent stem cell (hiPSC)-derived skin organoids (SKOs) generate sweat and sebaceous glands when differentiated using air-liquid interface conditions. (IMAGE)
Caption
Human induced pluripotent stem cell (hiPSC)-derived skin organoids (SKOs) generate sweat and sebaceous glands when differentiated using air-liquid interface conditions. a) Representative bright-field images of SKOs generated from the P112 hiPSC line on the transwell at Day 90. Red arrows indicate the hair follicles (HFs), and black asterisks indicate the cartilaginous parts. b and c) Representative hematoxylin and eosin (H&E) staining images, from the same SKOs shown in part a, confirm the presence of hair shafts (HS, dashed arrows), dermal papilla (DP), and sebaceous glands (blue arrows). c) Representative bright-field images of SKOs generated from the P112 hiPSC line on the transwell at Day 120, red arrows indicate the HFs. e and f) Representative H & E staining images, from SKOs confirm the presence of HS, DP (dashed arrows), and sweat glands (black arrowheads). g-k) Representative immunostaining images of SKOs on transwell. Dashed yellow boxes indicate the magnified regions. g, and h) Keratin 17 (KRT17) staining confirms the presence of HFs within the SKOs as indicated by white asterisks. Neuron specific class III beta-tubulin (TUJ1) staining confirms the innervation of the dermal layer at Day 120. i, and j) Immunostaining analysis confirms the presence of smooth muscle actin (SMA) positive arrector pili muscles and Keratin 20 (KRT20) positive Merkel cells next to the HFs. The blue color indicates cell nuclei stained with 4′,6 diamidino-2-phenylindole (DAPI). In parts i and j, the white asterisks, yellow arrowheads, and white arrowheads indicate HFs, Merkel cells, and arrector pili muscles respectively. Scale bars are 500 µm for the whole organoid sections in parts a, b, d, e, g, h, i, j, and k, and 100 µm for parts c, f, and inserts in parts g, h, i, j, and k.
Credit
Burns & Trauma
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