241219-Tominaga-Promoter-Illustration (IMAGE)
Caption
Kobe University bioengineers describe three design principles for efficient and reliably controllable yeast promoters, the genetic switches that regulate the artificially introduced genes necessary for the production of useful chemicals by the cell. First, if researchers not only need large amounts of the product but also the ability to switch the production on or off at will, they should introduce multiple copies of the regulatory elements enabling this within the promoter. This reduces leakiness and increases the productivity. Second, the distance between promoter elements should be as small as possible to enhance the productivity even more. And third, the promoter should be insulated from surrounding DNA by including extra DNA before it to further reduce leakiness.
Credit
Kobe University
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License
CC BY