STING signaling pathway is activated in 0.1% DDC cholestasis mice (IMAGE)
Caption
(A) Schematic illustration of the generation of C57/6J mice model of cholestasis. The experimental procedure for investigating the protective role of C170 in mice administered a 0.1% DDC diet at week 8 for 14 days. Intraperitoneal injections of corn oil or 10M C170 were administered daily to the treatment group mice (Chow Oil n = 4; Chow C170 n = 5; DDC Oil n = 7; DDC C170 n = 6). (B, C) Representative images (original magnification: 20×) and quantitative analysis of immunofluorescence staining for STING (yellow) and F4/80 (red). (scale bar: 50 µm). (D) Western blot of IFN-β in mouse livers. Expression levels were normalized to those of GAPDH. (E) Quantitative analysis of qPCR (top panel) and ELISA (lower panel) data for TNF-α, IL-6, and IL-1β. Data presented as mean ± standard deviation (SD). *p < 0.05, **p < 0.01. STING, stimulator of interferon genes; DAPI, 4′,6-Diamidino-2′-phenylindole; IFN-β, interferon-beta; GAPDH, glyceraldehyde -3-phosphate dehydrogenase; DDC, 3,5-diethoxycarbonyl-1,4-dihydroxychollidine; C170, a STING inhibitor; IFN-β, interferon-beta; IL-6, interleukin-6; TNF-α, tumor necrosis factor-alpha; IL-1β, interleukin-1 beta.
Credit
Jin Chai, Can-E Tang, Yan Guo
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted.
License
CC BY-NC