Phenotype produced by transient overexpression or silencing of FvRLP2 in strawberry fruits and Arabidopsis plants. (IMAGE)
Caption
Phenotype produced by transient overexpression or silencing of FvRLP2 in strawberry fruits and Arabidopsis plants. (A) Lesion development after inoculation of strawberry fruits with Botryti cinerea. DAY-1 indicated the first day after fruit were injected with Agrobacterium containing overexpression or silencing constructs of FvRLP2. DAY-3 indicated the first day after inoculation with B. cinerea. AS, silencing vector; EV, overexpression empty vector; OE, overexpression vector. (B) Measurement of lesion area using ImageJ. (n = 10, Student’s t-test, *P < 0.05 and **P < 0.01). (C) Relative expression levels of FaPR1 in fruit from different treatments (n = 10, Student’s t-test, *P < 0.05 and **P < 0.01). (D) Leaf phenotypes of transgenic Arabidopsis overexpressing FvRLP2 and wild-type control plants during infection by B. cinerea. (E) Trypan blue staining revealed cell death at 48 h after infection. (F) Measurement of lesion area using ImageJ (n = 10, Student’s t-test, *P < 0.05 and **P < 0.01). (G) Relative expression levels of AtPR1 in the transgenic lines, respectively (n = 10, Student’s t-test, *P < 0.05 and **P < 0.01).
Credit
Horticulture Research
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