CaSLR1 regulates the expression of cellulose, hemicellulose, and lignin biosynthesis related genes in pepper stems. (IMAGE)
Caption
CaSLR1 regulates the expression of cellulose, hemicellulose, and lignin biosynthesis related genes in pepper stems. (a) The expression-related heatmap of genes involved in the cellulose biosynthesis pathway and cell wall structural proteins. (b) The expression-related heatmap of genes involved in the hemicellulose biosynthesis pathway. (c) The expression-related heatmap of genes involved in the lignin biosynthesis pathway. (d) qRT-PCR analysis verified the down-regulated genes related to SCW formation pathways using RNA-seq. The results were expressed as the mean ± SE (n = 3). **P < 0.01; ***P < 0.001, as determined by the Student's t-test. (e) A hypothesis suggests that CaSLR1 regulated the stem lodging resistance. In WT, CaNAC6 binds to SNBE elements in the promoter of CaSLR1, thereby inducing its transcription. As a result, the stem accumulated cellulose, hemicellulose, and lignin, which promotes the deposition of the SCW and increases stem strength, enabling the stem remain upright. In contrast, in the mutant slr1, a deletion of CaSLR1 and its promoter abolished the binding of CaNAC6 to the SNBEs. The transcription of CaSLR1 was inhibited, and the accumulation of cellulose, hemicellulose, and lignin in stems was reduced. Accordingly, it resulted in the thinning of the SCW, weakening of stem strength, and finally, stem lodging. ‘×’ marked that the effect was cancelled. CESA: cellulose synthase; COBL4: COBRA-like 4; IRX9: irregular xylem 9; XTH30: xyloglucan endotransglucosylase protein 30; CCoAOMTL: caffeoyl-CoA O-methyltransferase 1ike; laccase 2 L: laccase 2-like; 4CL1: 4-coumarate-Co-A ligase 1; CAD1: cinnamyl alcohol dehydrogenase 1; 4CCL7: 4-coumarate-Co-A ligase like 7; AAE2: Acyl-activating enzyme 2; ACSL4: Long chain acyl-CoA synthetase 4-like; IRX15LL: IRX15 − LIKE − like.
Credit
Horticulture Research
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