Fig. 1. CRISPR-based genome editing in probiotics. (IMAGE)

Nanjing Agricultural University The Academy of Science

Fig. 1. CRISPR-based genome editing in probiotics.

Caption

(A) Integration of ldh gene into chromosome via the endogenous II-A CRISPR-Cas system in P. acidilactici. L, left arm; R, right arm; ori, replication origin; crRNA, CRISPR RNA; tracrRNA, trans-activating crRNA; marker, screening marker. (B) CRISPR/Cpf1-assisted multiple genes editing in B. subtilis. A mutation of Natronobacterium gregoryi Argonaute (NgAgo) was used to improve the homology recombination efficiency. (C) Gene editing based on δ sites of Ty elements in the yeast genome. (D) C·G-to-T·A amber mutation based on the CRISPR-cytosine BE in Bifidobacterium. APOBEC-1, a member of activation-induced cytidine deaminase/APOBEC nucleic acid cytosine deaminase family.

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BioDesign Research

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