Comparison of one-photon (1P) and two-photon (2P) brightness and sensitivity of fluorescent voltage indicators. (IMAGE)

SPIE--International Society for Optics and Photonics

Comparison of one-photon (1P) and two-photon (2P) brightness and sensitivity of fluorescent voltage indicators.

Caption

Comparison of one-photon (1P) and two-photon (2P) brightness and sensitivity of fluorescent voltage indicators. (a) Embryonic kidney cells were sequentially illuminated with wide-field 1P light in steps of increasing intensity and then by spiral scan 2P steps of increasing intensity. (b) Example cell expressing a genetically encoded voltage indicator. The 2P spiral scan pattern is shown in red, and the analysis for the region of interest is shown in green. Scale bars = 5 μm. 

Credit

F. P. Brooks III, H. C. Davis, et al., doi 10.1117/1.NPh.11.3.035007.

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