The CRISPR-Cas system combined with multiple signal amplification strategies can detect tumor exosome components (IMAGE)

Xia & He Publishing Inc.

The CRISPR-Cas system combined with multiple signal amplification strategies can detect tumor exosome components

Caption

(a) Utilizing HCR and CRISPR-Cas12a double amplification technique, a highly sensitive apta-HCR-CRISPR method was devised for the detection of TEV protein. (b) A novel CRISPR-Cas12a/Cas13a approach, activated by DNAzyme walkers, is suggested for the concurrent identification of exosomal proteins such as SAA1 and clotting FV. (c) A fluorescence aptasensor was created to detect NPC-derived exosomes by utilizing a combination of MNPs, TdT, and CRISPR/Cas12a. (d) An innovative biosensor, incorporating MNPs, CSDR, and CRISPR/Cas12a, was created to identify the exosome miR-21 in lung cancer. Cas12a, CRISPR-associated protein 12a; Cas13a, CRISPR-associated protein 13a; CD, Cluster of Differentiation; CRISPR, Clustered Regularly Interspaced Short Palindromic Repeats; crRNA, CRISPR RNA; DL, Deep Learning; EVs, Extracellular Vesicles; FQ, Fluoroquinolone;PD-L1, Programmed Death-Ligand 1. (a) Copyright 2020, Theranostics. (b) Copyright(2023), with permission from Elsevier; (c) Reproduced with permission from Springer Nature.(d) Copyright 2022, Multidisciplinary Digital Publishing Institute.

Credit

Shan-Wen Hu, Qi-Ling Yang

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