Alignment of CENH3 homologs and localization of CsCENH3 protein and ChIPed DNA on cucumber chromosomes. (IMAGE)

Nanjing Agricultural University The Academy of Science

Alignment of CENH3 homologs and localization of CsCENH3 protein and ChIPed DNA on cucumber chromosomes.

Caption

Alignment of CENH3 homologs and localization of CsCENH3 protein and ChIPed DNA on cucumber chromosomes. a Multiple alignment of CsCENH3 (C. sativus), NtCENH3 (Nicotiana tabacum), AtCENH3 (A. thaliana), OsCENH3 (Oryza sativa) and ZmCENH3 (Zea mays). The protein structure is shown below the sequence. The peptide sequence used to generate anti-CsCENH3 is highlighted in a red box. b–g Immunostaining localization of CsCENH3. Interphase nuclei and metaphase chromosomes (blue) were stained with 4′,6-diamidino-2-phenylindole (DAPI). Immunofluorescence signals (c and d, f and g) are visible within the nucleus (d) and in the centromeric regions of the chromosomes (g). h–j FISH signal of DNA precipitated by ChIP using anti-CsCENH3 antibody. Somatic metaphase chromosomes (h and j) that were hybridized to CsCENH3 ChIPed DNA probes. FISH signals (i and j) are visible in the centromeres (j). Scale bars: 10 μm.

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Horticulture Research

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