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Separating Ca2+ signals from background noises in confocal line-scan images of atrial myocytes. A Typical normalized confocal line-scan Ca2+ images of atrial myocytes in the presence (upper) or absence (lower) of 10 μmol/L tetracaine, for which the histograms of pixel fluorescence were fitted into modified Gaussian curves in the right panel. The blue arrow indicates the difference between raw and fitting traces, which represents the signal components. B Statistics of Fsignals and Rsignals calculated from the histogram and corresponding fitting curves of line-scan Ca2+ images of atrial myocytes from the WT mice in the presence or absence of tetracaine, n = 10 atrial myocytes from three mice per group
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Xu F, Li JJ, Yang E, Zhang Y, Xie WJ
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