Colletotrichum fructicola is a non-adapted fungus in Nicotiana benthamiana. (IMAGE)

Nanjing Agricultural University The Academy of Science

Colletotrichum fructicola is a non-adapted fungus in Nicotiana benthamiana.

Caption

Colletotrichum fructicola is a non-adapted fungus in Nicotiana benthamiana. A Disease lesions of pear fruits after C. fructicola inoculations. Conidia of C. fructicola were inoculated on ‘Dangshansuli’ pear (Pyrus bretschneideri Rehd.). Conidia buffer was infiltrated as a negative control. Disease lesions were observed and photographed every 12 hours post inoculation (hpi) within 48 h. B Phenotype of N. benthamiana leaves after inoculation of C. fructicola conidia. The leaves were inoculated with C. fructicola conidia by infiltration, and photographs showing both the front and the back sides were taken at 120 hpi. C Relative fungal biomass after inoculation of C. fructicola on pear host and N. benthamiana nonhost. Relative biomass was quantified by transcription-quantitative polymerase chain reaction (RT-qPCR) to measure the ratios of C. fructicola to pear or N. benthamiana DNA. PbrTubulin and NbActin were used as the internal references in pear and N. benthamiana, respectively. Differences were determined with Student’s t-test. Values are shown as means ± SD. *P < 0.05; **P < 0.01.

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Horticulture Research

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