Super-multicolor labelling of neuronal circuits with seven fluorescent proteins (VIDEO)
Caption
Mouse cortical layer 2/3 pyramidal neurons were labeled with 7-color Tetbow. A combination of 7 fluorescent proteins (mTagBFP2, mTurquoise2, mAmetrine1.1, mNeonGreen, Ypet, mRuby3, tdKatushka2) was used to visualize the dense wiring of neurons. The 7-channel images were then analyzed by the QDyeFinder program to reveal the wiring patterns of individual neurons.
Credit
Kyushu University/Takeshi Imai
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