An illustrated outline of the EarlyTect Bladder Cancer Detection (mePENK-LTE-qMSP assay) and its clinical applications (IMAGE)

Elsevier

An illustrated outline of the EarlyTect Bladder Cancer Detection (mePENK-LTE-qMSP assay) and its clinical applications

Caption

Urine DNA is extracted from the exfoliated cells in urine collected from hematuria patients. Bisulfite conversion differentiates the methylated PENK sequences by converting unmethylated cytosine residues to uracil. The detection of PENK methylation in urine DNA involves streamlined linear target enrichment (LTE) followed by quantitative methylation-specific PCR (qMSP), all within a single closed tube. The presence of PENK methylation indicates an increased probability of bladder cancer, warranting additional confirmation through cystoscopy for early detection or surveillance of bladder cancer.

Credit

Promis Diagnostics, Inc.

Usage Restrictions

Please include attribution.

License

Original content

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.