Morphological and histological observation of bulbil development. (IMAGE)
Caption
Morphological and histological observation of bulbil development. A Phenotypes of bulbil development in leaf axils from the first day of planting and continuing for 60 days. All white scale bars represent 1 cm. B Histological observations of bulbil formation. (i)–(iii) Epidermis cells of leaf axils underwent anticlinal and periclinal division, referred to as the ‘No bulbil’ stage. (iv) Apparent convexity appeared on the leaf axil, referred to as the ‘White dot’ stage. (v)–(vi) White bulbil dots further developed into mature bulbils with scales. All black scale bars represent 200 μm. AM, axillary meristem; LA, leaf axil; LP, leaf petiole; MS, main stem; SAM, shoot apical meristem; SC, scale. C, D Diagrammatic representation showing the distribution of bulbils in 30 independent plants on day 60 after planting: growth stages of bulbils (C) and number of bulbils per leaf axil (D). Each column represents a plant, and each box represents the type of bulbil. L1 represents the first leaf axil of the plant, and L means ‘leaf axil’. E Proportion of number of bulbils per leaf axil. Thirty biological replicates were performed. F, G Correlation between number of bulbils and plant height (F) and correlation between number of bulbils and number of leaves (G). Thirty biological replicates were performed. Pearson’s correlation (r) analyses (P<0.05) and scatter plots show the data distribution.
Credit
Horticulture Research
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