Exploring the effect of H2O2 eustress at single-cell level using hopping probe scanning ion conductance microscopy (IMAGE)
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Left: The study utilizes hopping probe scanning ion conductance microscopy (HPICM) to non-invasively investigate the cellular morphology and mechanical properties of individual colorectal cancer Caco-2 cells, offering precise imaging and characterization without physical contact. Middle: Highly sensitive Pt-functionalized carbon nanoelectrodes enable the measurement of dynamic changes in extracellular-to-intracellular H2O2 gradients of individual Caco-2 cells under H2O2 eustress conditions. Right: The study observed that Caco-2 cells responded to H2O2 eustress at 0.1 mM by increasing cellular F-actin-dependent stiffness, but this stiffness decreased at 1 mM. Interestingly, this eustress-induced stiffness positively regulated AKT activation while negatively influencing the expression of the H2O2-scavenging enzyme GPX2. These findings reveal a novel interplay between physical properties and biochemical signaling in cancer cells' antioxidant defense, shedding light on the exploitation of H2O2 eustress for survival at the single-cell level.
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© 2024 Science China Press. Published by Elsevier B.V. and Science China Press
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