Fig. 5. Engineering the GAL system to function in YPD medium for an improved carotenoid storage. (IMAGE)

Nanjing Agricultural University The Academy of Science

Fig. 5. Engineering the GAL system to function in YPD medium for an improved carotenoid storage.

Caption

(A) PEST fusion to Gal80 for copper-induced GAL expression in YPD media. Copper sulfate (20 μM) was used to induce the carotenogenic genes under the control of copper-inducible GAL system. (B) Carotenoid contents of engineered yeasts in YNBD and YPD media at 72 h. (C) Growth curves of JS-BE5 and JS-BE5-PEST cultivated in YNBD and YPD media. Strain JS-BE5-PEST is derived from JS-BE5 with PEST-Gal80N. (D) Representative HPLC results showing the abundance of carotenoids produced by JS-BE5-PEST. P values were obtained from two-tailed unpaired Student’s t test. ***P < 0.01. Error bars indicate the SDs of three independent experiments.

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BioDesign Research

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