Fig. 1: Example application of the developed method. (IMAGE)

Kanazawa University

Fig. 1: Example application of the developed method.

Caption

The predicted orientation of the Cas9 protein on the AFM substrate is shown, and a simulated AFM image calculated in the scanning view perspective shows remarkable agreement with previous HS-AFM imaging. HS-AFM can reliably visualize functional relative motions of target DNA and Cas9 because the entry and exit gates of the DNA strand, which is located in a tunnel within the Cas9 structure, are not blocked by contacts with the APTES-mica substrate.

Credit

2023 Amyot, Nakamoto, Kodera and Flechsig.

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Credit must be given to the creator.

License

CC BY

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