Cryo-EM structure of PvrA-DNA complex (IMAGE)
Caption
A, modelling the overall structure of the PvrA-DNA complex into the cryo-EM density map. The overall structure contains three PvrA dimers: two PvrA bound to the both sides of the DNA, and one PvrA bound to the middle region of the DNA. B, Structural superposition between the middle PvrA with the side PvrA, highlights the different conformational state adopted by distinct DNA binding site in the complexes. The inset shows that the axis of HTH-domain tilts upwards by an angle of 3° in the middle PvrA state relative to the side PvrA conformation. C, Multiple PvrA binding leads to DNA distortion, with the side PvrA binding site (green sphere) and the middle PvrA binding site (blue sphere) within a single DNA duplex, forming a distinct 15.4° angle. D, The two DNA double helices rearrange upon binding to PvrA, and a sharp angle of about 20.4° was formed between the DNA duplexes, and this well-defined topography facilitated the binding of PvrA.
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