Overview of establishment of BC-PD-L1 (IMAGE) KeAi Communications Co., Ltd. Caption I Overview of establishment of BC-PD-L1. (A) Overview of the models of BCCs for potential applications in prediction of prognosis in bladder cancer. Urine/tumor samples were collected from BLCA patients for primary bladder cancer cell culturing. PD-L1 expression levels of BCCs before and after IFN-γ stimulation were measured by FCM and used for prognosis and ICI response prediction. (B) Pie charts showing the classification of all urine/tumor-provided patients based on sample source, gender, pathology group, and disease status. (C) Levels of PD-L1 expression on BCC cell surface before and after IFN-γ treatment measured by FCM analysis. (MFI mean ± standard deviation). MFI, median fluorescence intensity. Credit Jiaqi Wang, et al. Usage Restrictions Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted. License CC BY-NC-ND Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.