Data-Independent Acquisition-based global phosphoproteomics reveal the diverse roles of Casein kinase 1 in plant development (IMAGE)
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A quantitative DIA-based phosphoproteomics were conducted with Arabidopsis CK1 related materials and phosphopeptides were enriched and substrate motifs of CK1 were enriched among phosphopeptides. One candidate substrate, a CCCH-type zinc finger protein, C3H17, was selected from phosphoproteins and proved being phosphorylated by CK1 to promote the protein stability and transactivation activity to regulate embryo development. Considering the conservativeness of CK1, substrate motifs were used to predict new candidate substrates in Arabidopsis, rice, mice and humans, which greatly facilitate investigating the roles of CK1-mediated phosphorylation in different species.
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